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Cisplatin cck8

WebCisplatin-induced acute kidney injury in mice was established by intraperitoneal injection of cisplatin. The levels of urea nitrogen and creatinine in serum were measured， renal pathology change was assayed by HE staining， qRT-PCR and Western blot were used to detect the expression levels of PIM1 mRNA and PIM1 protein. WebJan 12, 2024 · Parental and cisplatin-resistant HeLa cells were cultured in the presence of 1.0 μg/mL cisplatin in 96-well plates and stained with cell counting kit-8 (CCK8) at indicated time. Values are presented relative to cell viability on day 0. * p < 0.05, ** p < 0.01 compared with the parental cells; ( F ) Western blotting analysis of NHERF1 ...

[miR-29b Reduces Cisplatin Resistance of Gastric Cancer Cell by ...

WebSep 30, 2024 · Cisplatin was the first platinum-based agent shown to have anti-tumor properties. 2 Cisplatin-based combinations remain first-line therapy for a number of solid tumors including lung, ovarian, and esophageal cancer. 3 It induces DNA damage through DNA cross-linking, thus interfering with the fundamental processes of DNA replication … WebCCK8 assay was performed to assess cell proliferation and chemosensitivity of cisplatin-resistant A549/DDP and H1299/DDP cells. Colony-forming assay was utilized to detect colony numbers. Cell migration and invasion ability were measured by transwell assay. The protein levels of LRP, Pgp, MRP1, and SIX1 were examined by Western blot assay. ipc integrated phase classification https://bjliveproduction.com

Cisplatin - an overview ScienceDirect Topics

WebMar 27, 2024 · Cisplatin-induced cell killing was analyzed by CCK8 assay. Intracellular ROS levels were detected by fluorescence-based flow cytometry analysis. In vitro overexpression and knockdown of GPX1 expression were performed using GPX1 expression vector and siRNA approaches. Web瑞禧生物提供科研级cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购cisplatin-PEG-NPC顺铂-聚乙二醇-硝基 ... Web目的 探讨姜黄挥发油(TVO)联合顺铂对人皮肤鳞状细胞癌(鳞癌)A431细胞增殖、凋亡的影响及机制.方法 取对数生长期A431细胞,用5、10、20、40、80 mg/L TVO处理,对照组用含有1％二甲基亚砜(DMSO)的DMEM高糖培养基处理,24 h后,CCK8法检测细胞增殖情况.另取部分A431细胞,分为4组,分别用含1％ DMSO的DMEM高糖培养基(对照 ... ipc interagency

[miR-29b Reduces Cisplatin Resistance of Gastric Cancer Cell by ...

Category:Nrf2 induced cisplatin resistance in ovarian cancer by …

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Cisplatin cck8

IJMS Free Full-Text NHERF1 Enhances Cisplatin Sensitivity in …

http://www.rx-8.com/chanpinzhongxin/203370.html WebMay 28, 2024 · Non-Small Cell Lung Cancer (NSCLC) patients harboring EGFR activating mutations, such as in-frame deletions in exon 19 (Ex19del) or missense mutation in exon …

Cisplatin cck8

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WebJun 11, 2024 · Cck8 colorimetric assay was used to detect the sensitivity of cells to anticancer drugs, cell viability and proliferation in response to drug treatments in vitro. They were also used to determine the concentration of the drug that inhibited cell growth by 50% (IC 50) after 24 h of treatment. WebMethods: We use CCK8 assay to show synergistic cytotoxicity of COE and cisplatin. Then, PI single staining and FITC-Annexin V/PI double staining were used to observe apoptotic cells through flow cytometry. The proteins of caspase signaling pathway were examined by Western blotting.

WebJan 1, 2024 · New therapy targets are urgently needed to reverse DDP resistance. Methods: The protein levels of these molecules were analyzed by Western blotting and immunofluorescent staining. Cell proliferation was detected by CCK8 and MTT assays. Cell migration and invasion were analyzed by wound healing assay and Transwell assays. WebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was …

WebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. Transfection of wild-type p53 gene enhanced the ... WebJul 4, 2024 · To investigate whether miR‐320a regulates laryngeal carcinoma cell cisplatin resistance, miR‐320a overexpression HEp‐2 cells were treated with various concentration of cisplatin. CCK8 assays demonstrated that miR‐320a overexpression significantly inhibited HEp‐2 cell viability (Figure 4 E).

WebCCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of DNA repair-related proteins. Transwell assay was used to detect cell invasion. Results DNA repair was positively associated with the …

ipc insulationWebOct 22, 2024 · Cell viability was analyzed by the Cell Counting Kit-8 (CCK8). The relationship of Nrf2 and CD99 was assessed by dual-luciferase reporter gene assay and chromatin immunoprecipitation (ChIP). Bioinformatics analysis was performed to search for the downstream gene of CD99. open the eye of my heart lyricsWebAug 8, 2024 · a Before and after treatment with the supernatants of Tregs, cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM) was analyzed by the CCK8 assay. b With different concentrations of Treg supernatants (supernatant frequency of Treg/tumor cell = 1:1, 1:2 and 1:3), cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM ... ipc in supply chainWebFeb 28, 2024 · CCK8 was used in ( a) A549 cells and ( b) H460 cells with different concentrations of cisplatin. ( c) CCK8 was used in A549 and H460 cells transfected with miR-494 or controlled with... ipc international limitedWebSep 9, 2016 · The CCK8 assays revealed that, compared to scramble vector, PD-L1 depletion (Fig 2C, left panel) induced more cell growth arrest in the presence of cisplatin (Fig 2C, right panel), suggesting that PD-L1 abrogation restored the sensitivity of H69R and H82R to cisplatin treatment. Thus, PD-L1 upregulation might be required for H69R and … ipc international project consultingWebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … open the eyes my heart chordsWebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … ipc international patent classification