Web这篇7+文章提供了新指南!. - 知乎. 泛素化研究很难?. 这篇7+文章提供了新指南!. 蛋白作为生命功能的主要的执行物质,其功能研究一直备受青睐,蛋白翻译后修饰(PTM)更是众科研人的聚焦点,所以相关研究也如雨后春笋般纷纷冒出。. 而今天我们就以一篇 ... WebJan 12, 2024 · Mix carefully and allow to stand at room temperature for 10 min to precipitate the RNA. Centrifuge the mixture at 12,000 x g for 10 min at 4 °C. Remove the supernatant and wash the RNA pellet by adding 1 ml of 75% ethanol, vortex and centrifuge at 7,500 x g for 5 min at 4 °C. Briefly air-dry the RNA pellet for 5-10 min.
Analysis of Protein Stability by the Cycloheximide Chase Assay
WebCycloheximide (CHX; mol. wt. 281.35) is a beige colored, crystal powdered, water-soluble, semisynthetic compound, ... An alternative is the cycloheximide chase assay, where global protein synthesis is blocked using the translation elongation inhibitor cycloheximide. With this method, the clearance of the protein of interest can be ascertained ... WebD , E qRT–PCR analysis of AURKA mRNA after transfecting siHMMR (#1, #2) or negative control in DU145 ( D ) and PC-3 ( E ) cells. F Western blotting analysis of AURKA levels after treating cells (vector and HMMR) with CHX at 0, 3, 6, and 9 h to assess the degradation ratio of AURKA. CHX concentration: 50 μg/ml, left panel: DU145, right panel ... ipaf and pasma training
Cycloheximide - an overview ScienceDirect Topics
Web因为CHX是蛋白合成抑制剂,做蛋白降解实验首先得确保不再产生新生蛋白,然后才能观察蛋白降解情况(换一种说法是研究不同情况下蛋白的半衰期)。. CHX,中文名为放线菌 … WebCycloheximide is supplied as a lyophilized powder. For a 10 mg/ml stock, carefully weigh out and reconstitute 50 mg in 5 ml DMSO or EtOH. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µg/ml for 4-24 hours. Soluble in DMSO, EtOH, or MeOH. Wear personal protective equipment. WebCycloheximide (CHX; mol. wt. 281.35) is a beige colored, crystal powdered, water-soluble, semisynthetic compound, ... An alternative is the cycloheximide chase assay, where … open season play a sound book